Incident at Reception Desk Essay Example | Topics and Well Written Essays - 500 words

Incident at Reception Desk - Essay Example I did not use any negative terms, instead I said, When I told him that because of regulations, it was not possible to provide him with what he wanted at this time, he slammed his hands quite forcefully down on the counter top and pushed his body forward, his face really close to mine. I took a step back, he was very angry and his body language was threatening. At this point, he began to insult me in quite a low, menacing tone, calling me a "stupid bureaucratic, pen-pushing moron with no initiative!" whore," and "brainless f..ing bitch". He got to the door, and shouted back that I would regret being so unhelpful, that he would be suing me for my failure to provide the correct public service he was entitled to. He also said I would be hearing from his uncle, P. Bull Terrier, that "even a fool like you must have heard of him!" He banged the door really hard as he left. Two of the members of the public came to the desk and were real kind. They gave Joelly their details and offered to be witnesses in support of the fact that I had behaved politely and correctly throughout. The incident left me shaken and rather nervous, but after discussing it with you and writing it down, this has helped to clarify things. I feel certain that I carried out my duties correctly and have done nothing to bring trouble or disrepute on the department. I look forward to your response.

Essay Writing Layout - Effective Layout For Online Students

Essay Writing Layout - Effective Layout For Online StudentsIf you're an aspiring essay writer and wondering what the most effective essay writing layout would be, then keep reading. You're going to discover what I consider to be one of the most effective essay writing layouts for any online student. You'll see that the layout is free, uses very few fonts, and is very easy to follow.Make no mistake, this layout is for those who have no time to make their essays on their own. This layout, when used with the right information, can make your essay very effective. After all, nobody wants to read a hundred word essay that they find boring. In the best case scenario, they get one-word sentences out of it. That's it.Writing an essay for yourself, no matter how you do it, can be an easy task, if you follow the right steps. But there are too many people who fall victim to writing for themselves. They really don't want to think, they want to just copy someone else's essay and copy and paste it into a spreadsheet. Now if they'd done that, they would have had to pay for the privilege.The first thing you should do if you want to write your essay yourself is to study up on things like grammar and punctuation. This helps you better understand how your essay should be formatted and that you should follow certain rules in your writing. As a result, you won't have any difficulty when it comes to getting your essay on paper. It also means that you won't have to go through the process of drafting your essay, which can take a long time and is very tiring.Another way to make sure that you're not going to do things yourself, is to make use of the free style essay writing layout. Although it costs nothing, I would advise you to not to use it unless you are completely sure that you have anexcellent writing style. Your main objective is to make your essay look professional and appealing to the reader.An important tip to remember is to make sure that you have a very simple thesis. Keep it short, concise, and to the point. I've found that just leaving out my entire thesis would make my writing look like a large chunk of text. The end result will be that you'll waste more time looking for the right words to use.By using these two layouts, you can ensure that you get your essay on paper, but also that you don't waste your time trying to work out the rules for proper grammar and punctuation. I hope that by now you've realized how useful it is to use the right layout for your essay.

The Secret of Prison Research Paper Topics That Nobody Is Talking About

The Secret of Prison Research Paper Topics That Nobody Is Talking About Life After Prison Research Paper Topics Writing a research paper even though may appear challenging is a considerable portion of routine student life. All our writers have top-of-the-line education and can assist you, since we've got double excellent check, and you won't ever receive a paper that embarrasses you. Our academic writers are sure to compose impeccable papers. Corrections topics research papers and essay topics may change depending on a type of student. In the last year of college, research paper topics need to be more specific and the research needs to be in-depth. You've searched here and there to learn a proper topic for research but all your efforts have gone in vain. You should attempt to pick a topic for your research subject in line with the instructions of your professor. What to Expect From Prison Research Paper Topics? If a teacher doesn't assign a particular research paper topic, it usually means that you could think of your own idea. Not all research paper topics are made equal, and you would like to be sure you decide on a fantastic topic before you commence writing. Following are a few of the advised sociology essay topic for those students that are unable to decide on a great topic for their assignment. Even if a particular research paper topic is getting a great deal of buzz at the moment or other men and women seem interested in writing about it, don't feel tempted to make it your topic if you don't genuinely have some type of interest in it also. The entire research should be constructed around or from the subject. There's well-known that you aren't going to have the ability to compose a superior insightful research paper if you're not interested in the subject overall and in this issue particularly. Attempting to compose a research paper on a topic that doesn't have a lot of research on it's incredibly hard, so before you choose a topic, do a little preliminary searching and be certain you will have all the info you have to compose your paper. A History of Prison Research Paper Topics Refuted When you are concerned about writing research paper, it can cause you to be stressed and not to have the ability to perform in the simplest way throughout different regions of your lif e. Not only may not bomb the paper, but you may also begin seeing your grades drop in different areas because you're stressed. The Importance of Prison Research Paper Topics Therefore, usage of alternative sentencing will assist in stabilizing the prison populations. Researching any issue about government or laws may get overwhelming because of the intricacy of the issues and even on account of the wording of some laws. When it has to do with understanding the way the prison system in the united states has failed, it looks like there's a sequence of events that ultimately lead a prisoner farther down the path of further unlawful activity in place of rehabilitation. Naturally, one large portion of understanding the prison process is knowing its roots and development with time. Specifically, it is all about the rules of citation, for instance, an APA research paper format. Each article ought to have appropriate structure. Finding research paper topics on the web is relatively uncomplicated and your search should yield a variety of ideas. If you still feel you need help, even if you've managed to decide on a topic, you may always employ a custom writing service to assist you produce a fabulous research paper of which you'll be proud and will guarantee you a nice mark. When you own a topic and a list of references, you might want to write down all of the critical points you should include, developing a plan for your justice paper that may be developed into the very first draft just by describing each point. Once your very first draft is ready, you can take a rest and allow it to sit for a small while. When you're back, you can get started working on the finished draft and its editing, paying attention to the details alongside formatting aspects. Based on the Prison Violence Prison violence is something which has been an ongoing issue and can be understood in many diverse forms. Women in the majority of the prisons undergo medical neglect. If it co mes to America's prison system it appears that there are lots of ironies engendered within the computer system. Some prisons make it possible for inmates to gain from educational programs to receive their high school diplomas or receive vocational training. Evidently, you won't have the ability to compose a research paper if sufficient information concerning the subject isn't offered. Choosing one of our suggested topics will enable you to commence writing a thriving research paper. After the topic is chosen, it would be helpful to make an outline so you could see whether you have sufficient information for good-structured research paper. When deciding on your research paper topic, you should make certain it is neither boring nor worn out. The more you comprehend the simpler it's for you to compose a thriving research paper. Writing a research paper requires a lot of time, therefore it is vital to opt for a research paper topic you will really delight in working with. Formatting a research paper is not a simple job. Writing a great persuasive research paper is a difficult job.

The Battle Over Act Essay and College Requirements and How to Win It

The Battle Over Act Essay and College Requirements and How to Win It You can appropriate some or each of the examples in it to utilize in your essay. Once you get your completed essay, be certain to tell all your friends what a fantastic service it is and what's the ideal place to obtain cheap essays. Now you are aware of how to compose a great ACT essay. A competently compiled admission essay will assist the applicant to concentrate on successful facets of his academic life and provide decent account of himself. Choosing Act Essay and College Requirements Is Simple Application for Admission and class e-schedules are offered on the internet. Services Cases can differ. Students could be accepted without the suggested variety of courses in a specific field on a case by case basis. If you want to get considered for admission to the Harriet L. Wilkes Honors College, you'll need to submit an extra application together with meet their admission criteria. Application for Admission and e-class schedules are offered on the internet. Check with the institution to find out whether it offers such classes. We understand that as an ECHS student, you aren't the very same as most incoming freshmen, therefore it is logical your admissions process should be a bit different too. You might decide to select the chance to re-take the ACT test to increase your subject scores. By doing this, students will have the choice of taking the test again in case the scores aren't satisfactory. Whenever you have the last score that you're delighted with, after that you can send only that score to all of your schools. For more thorough information regarding ACT target scores, have a look at the post on what's a great ACT score. Finding the Best Act Essay and College Requirements It's possible to take a look at their profiles to find out more. For instance, if you believe writing a case study or completing a data analysis is straightforward, critique you've never francais this before. What's more, you should do well to get a strong application. Download our free guide on the top five strategies you have to be using to enhance your score. It can be noted that almost all of these on-line classes are meant for working professionals. The present day malls and multiplexes also have made their appearance within this residential region of South West Delhi. In addition to its growing cultural and ethnic diversity, State University is turning into a master at developing a niche for each and every student. Online, it is not just the perfect place for me, requirements is the only place for me. These schools are considered among the very best of them, and you'll observe the standard score ranges for admission are much like those for larger top universities. No more than 1 year of ESL-type courses can be utilized to fulfill this requirement. Your colleges will get your scores in three to eight weeks, therefore it is better to select the test three or more mo nths before the admission deadline of the college of your pick. Del Mar College uses assessment scores to help you select the best courses to achieve your goals based on your present amount of reading, writing and math. Teach them what an excellent home life appears like. Often there's a public relations team in an advertising agency which aids business house build goodwill in the marketplace and also provide information regarding the business house and their goods and services in the industry. You've got to teach them not to get rid of track of the significant things. Look at each subject region and activity for each individual in your residence. The Organization scoring domain is a fairly easy one to succeed on in the event you comply with the protocol, so make sure that you nab your points here. This is essential for your testing strategy. Controversy erupted soon after the ACT introduced a revised essay-writing task in September that's being graded for the very first t ime on the exact same 36-point scale as the remainder of the test. If you own a mix of a math and lab science deficiency, you aren't admissible. I am involved in a lot of cycles of procrastination both big and small. These features are like those that hood studies the prior customers o what is the true subject of this sentence. You've got an extremely very good opportunity of raising your score, which will significantly boost your odds of getting in. If you exceed their requirements, you have a superb possibility of getting in. Finally, it is possible to also use the completely free tool from Cappex that will allow you to understand your opportunity of getting into a particular school. If you don't see your college on such list, they might be awaiting a last choice.

Decrease childhood obesity Research Paper Example | Topics and Well Written Essays - 3000 words

Decrease childhood obesity - Research Paper Example Unfortunately, the study has it that child obesity can lead to depression and poor self-esteem. Statistically, according to US’s department of health, there is a dramatic increase in number of children suffering from obesity. Considerably, it is noted that almost ten percent of children aged four and five are overweight, and the above number is double to that of twenty years ago. The research argues that obesity is a gender related disorder where, girls are the ones who suffer obesity more compared to boys. It is agreed that childhood obesity is an alarming disorder that needs immediate action. Therefore, to decrease childhood obesity, it is pertinent for all schools in the US to incorporate salad bars into the children’s lunch program. In so doing, those children will be able to access fresh vegetables and fruits, which in turn will develop healthier life from long eating habits (Linsley, Kane, & Owen, 2011.p.81). This essay tactically illustrates the policy recommendations for obesity in children, obesity regulations program and the US federal laws, assumptions and pertinent secondary recommendations to mention but a few, all converging to the childhood obesity issue. ... Although the shift may seem small, the size of the sample collected for the study makes it significant. Notably it is unbelievable to realize that each percent stated above points for almost 26,700 in the population study, which are either extremely obese or no longer obese. Forcibly, the Obama’s administration involving in the fight for obesity in children shows the severity of the disease among the US children who live in poverty. First lady Obama Michele has tirelessly fought the eradication of poverty through her message involving not only healthier diet but also physical activities. According to recent research, it is pertinent to note that the new trends in obesity levels indicate a modest progress of prevention of obesity in young children. The above study is pertinent for the implications of health risks for extreme obesity and obesity in young children. Therefore, the above passage outlines the importance of tackling obesity in children by the US government. It is evi dent that obesity can to some extent affect the young US generation undesired diseases that were supposedly to be for adults. Policy Recommendations for childhood obesity Controlling and preventing childhood obesity requires a multifaceted and wide programs staged by the communities as well as formulation of policies where parent will play a vital or critical roles. Pertinently one of the important elements that can regulate obesity involves the parents. Here, the children’s behaviors are affected by the eating habits of the parents, level of education as well as physical education. According to the study, it is noted that parents are role models and they influence to some extend the physical

Non-invasive Strategy for Isolating Cancer Cells

Non-invasive Strategy for Isolating Cancer Cells Here, we report non-invasive strategy for isolating cancer cells by autonomously propelled carbon nanotube (CNT) microrockets. H2O2-driven oxygen (O2) bubble-propelled microrockets were synthesized using CNT and Fe3O4 nanoparticles in the inner surface and covalently conjugating transferrin on the outer surface. Results show that self-propellant microrockets can specifically capture cancer cells. Self-propelled micro-motors have paved the way to exciting applications in biomedical field such as delivering drugs,[1] nanoscale transport and assembly,[2] motion-based biosensing disease markers and microrobotics.[3] The usage of such micromachines to transport diverse payloads is one of the next prospects for nanomotor development.[4-6] Particularly chemically powered micro-/nanomotors based on different chemical compositions and structures, that are capable of moving autonomously in the presence of hydrogen peroxide fuel are being given emphasis.[2,3,7,8] Among these, self-propelled microshuttle is lucrative for practical biomedical applications.[2-7]. Especially, fabrication of nano and micropropellant systems featuring specific cell recognitions in shortest time frame is highly anticipated and yet challenging. We report for the first time a chemically-powered CNT based magnetic micromachine for isolating and transporting cancer cells. Described microrocket technology could find a potential application as a bio-analytical micromachine for selective and rapid isolation of circulating cancer cells (CTCs). Detecting CTCs is a challenge due to the extremely low occurrence (10-100 per milliliter of blood) of CTCs among a large number of hematologic cells in the blood (109 mL-1).[10,11] Several strategies, involving immunomagnetic beads or microfluidic devices, have been designed for isolating and accounting CTCs from blood.[12-14] However, most of these approaches are limited by their slow rate and low CTC-capture yield.[13,14] Currently, only one technology is available commercially (CellSearch ®) to identify CTCs from the blood of cancer patients.[9] We recently reported rapid and specific isolation of CTC based on magneto-dendritic nanosystem [12] Here we show the preparation of bio-functionalized microrockets and their application for rapid isolation of cancer cells. Microrocket system consists of three functional components: (i) CNT, (ii) iron oxide (Fe3O4) nanoparticles for magnetic isolation, and (iii) Tf ligand (Tf) for specific targeting. Fe3O4 nanoparticles were loaded in the inner surface of CNTs as described previously with some modifications.[15] The unique advantages of the microrocket platform are: i) low density of microparticles ii) instantaneous propellant motion of microparticles (downward and upward) with an ability to ‘strike’ cancer cells in its path, iii) rapid capture (~5 min) of TfR-overexpressing (TfR+)cancer cells at the clinically relevant concentrations (approximately 1 CTC per 105 blood cells), iv) specific targeting ability due to presence of Tf ligand, which is generally used to capture cancer cells overexpressing TfR on their membranes, v) magnetic isolation of the captured cells owing to the presence of Fe3O4 nanoparticles and (v) large surface area and internal volume allows multiple components to be loaded onto the nanotube. We followed a multi-step process (Figure 1A) to synthesize the Tf-CNT-Fe3O4 platform. Figure 1A displays the TEM image of Fe3O4-CNTs. We observed that Fe3O4 particles deposited inside CNT had a uniform size of ~ 6 nm (see supporting information; Figure S1). Moreover, the image indicates the successful assembly of the Fe3O4 nanoparticles in the nanotubes with 4~8 nm i.d.Coupling of Tf on CNT-Fe3O4 composite was confirmed by Fourier transformed infrared (FTIR) spectroscopy (see supporting information; Figure S2). Further, Tf attachment quantified by a modified Bradford procedure was found to be ~ 0.2 mg of Tf per g of CNT-Fe3O4. The magnetic property of Tf-CNT-Fe3O4 particles was also visually evaluated in aqueous medium by placing it next to a permanent magnet (see supporting information; Figure S3). Figure 1B illustrates the self-propulsion of suspended Tf-CNT-Fe3O4 microparticles mimicking microrocket in a solution containing H2O2. Microrockets initially propelled instantaneously towards the bottom of the tube and gradually reverted direction upwards. We noticed that the speed of the Tf-CNT-Fe3O4 microrocket was strongly dependent on the H2O2 concentration (Figure S4). As expected, the microrockets display highest speed at the maximum H2O2 (8%) concentration tested in aqueous solution. The upward directional speed of the microrockets decreases gradually from 0.90 mm†¢s-1 (at 8%) to 0.68 mm †¢ s-1 (at 4%), and subsequently to 0.56 mm †¢ s-1 (at 1%) per unit area of the particles. We hypothesize that microrocket motion is influenced by the surrounding H2O2 concentration as it affects the rate of the Fe3+ reduction to Fe2+. Notably such chemically powered micromotors are commonly incompatible with the high ionic strength environment of biological fluids and extending the scope of such microrockets to physiological conditions is a key challenge. So we studied the ability of our microrockets to propel in biological fluid such as Dulbaco’s modified eagle medium (DMEM) cell media. Interestingly, the microrockets were found to propel efficiently even in DMEM containing 4% of H2O2. Figure 1B shows images of the microrocket at different vertical positions during its motion for a complete cycle. Initially, it was observed that microrockets, of smaller dimension, floated at the meniscus due to the low density and attached O2 bubbles, formed by Fe3O4 nanoparticle catalyzed decomposition of H2O2. Once the smaller bubbles dispersed, a downward movement was observed (Figure 1B) due to the gravitational force. In the meantime, more O2 bubbles formed and adhered to the Tf-CNT-Fe3O4 particle and in few seconds, the adhered O2 bubble grew larger by coalescence of several smaller bubbles. Eventually the total volume of the bubble was sufficiently high, so that the buoyancy force balanced the gravitational and viscous forces and the microrocket moved upward (Figure 1B).[16,17] Figure 2A shows the tracking trajectory of a microrocket in cell media, indicating a vertical motion. It is noted that the microrocket moved with high average speed of 0.38 mm †¢ s-1 in the downward direction and 0.62 mm †¢ s-1 in the upward movement per unit area of the particle (about 12 and 16 times its body length per second). This corresponds to a large driving force of over 231 and 300 pN, based on the drag force F=6πΠ¼rv, where v is the speed, and ÃŽ ¼ is the viscosity of the medium and r is the radius of the microrocket. Further, when the concentration of H2O2 in cell media was varied there was notable effect on the speed as shown in Figure 2B. The speed of the microrocket at 8% H2O2 concentration was ~1.9 and~1.4 times higher in the downward and upward direction than at 1% of H2O2 concentration. Also, the number of times the microrocket moved up and down changed. At high H2O2 concentration (8%) the total distance moved by the microrocket was ~2 times more than that at lower concentration (1%) (Figure S5 and S6). Moreover, it was also revealed that the speed of the microrockets was only slightly affected in DMEM. The speed of the microrocket was 10% less in DMEM cell media compared to aqueous media containing 4% H2O2. Partial blocking of Fe3O4 surface by adsorbed proteins and increased solution viscosity may be respon sible for this moderated speed. We validated the application of microrockets for selective and rapid isolation of cancer cells from a heterogeneous population by separating HCT116 cells from an artificial CTC suspension. We placed cell suspension in DMEM at the concentration of (1 x 106 cells mL-1) containing 4% H2O2. Tf-CNT-Fe3O4 microrockets were incubated in cell suspension for 5 min to target and isolate the HCT116 cells. We envisioned that Tf-functionalized microrockets could strike and selectively bind suspended HCT116 cells through the TfRs and finally transport them at the top of the tube from where they can be retrieved. Figure 3A illustrates the pick-up and transport of a cancer cell by a microrocket. On the other hand, Figure 3B shows cancer-cell-loaded microrocket. The force necessary for moving a relatively large (~16 mm) cancer cell is considerably high. The minimum force necessary for transporting such large cells at one body length per second in DMEM estimated from Stokes’ law is 1.88 pN. The high speed of the microrocket is slightly affected by the cell loading (e.g., decreasing from 0.62 to 0.50 mm†¢s-1 in cell media), reflecting its high towing force. We observed that Tf-CNT-Fe3O4 microrockets can efficiently pick-up and transport HCT116 cancer cells. We observed rapid action and selective targetability of Tf-CNT-Fe3O4 microrocket in capturing cells from a suspension. It also confirmed that the isolated cells remained normal for ~30 min and could be used for further studies (see supporting information; Figure S7). In addition, the HCT116 cell viability was also evaluated in DMEM containing 4% H2O2 after 1 hour. The study showed majority of the cells (78%) remained viable (Figure S7). Furthermore, in order to confirm that the specific interaction of Tf-CNT-Fe3O4 microrocket with TfR+ cells (such as, HCT116) is because of Tf, we used CNT- Fe3O4 microrocketwithout Tf to capture TfR+ cells (as a negative control). We found that no cells were attached to the CNT-Fe3O4 microrocket even after 5 min incubation. Hence, experiments with HCT116 confirm that after 5 min incubation, there is very little non-specific interaction of cells with the Tf-CNT-Fe3O4microrocket. In addition, to evaluate the effect of self propulsion of Tf-CNT-Fe3O4 microrocket on HCT116 capture, Tf-CNT (without Fe3O4) was used. It was found that Tf-CNT could capture only ~ 22% HCT116 cells (see supporting information; Figure S8). The study shows that self-propulsion of Tf-CNT-Fe3O4 microrocket plays a significant role in cell capture and isolation. Our final goal was to quantify the capture efficiency of microrocket while targeting cancer cells from an artificial CTC suspension. We spiked human peripheral blood mononuclear (hPBMC) cells with TfR+ GFP-labeled HCT116 cells in various hPBMC: HCT116 ratios (e.g. 1 x 103:1, 1 x 104:1 and 1 x 105:1). Cell suspensions of different ratios were incubated with Tf-CNT-Fe3O4 microrocket for 5 min prior to magnetic isolation. Following isolation, both the captured and the residual cell suspensions were imaged to estimate the number of captured and uncaptured HCT116-GFP cells in each sample. Assuming the number of captured HCT116-GFP cells to be NC, the number of uncaptured HCT116-GFP cells to be NU and following the method of Zheng et al, the capture efficiency (C.E.) for each dilution was estimated as:[18 (1) Figure 4A shows image of captured cells (green) attached to the Tf-CNT-Fe3O4 particles (black). As expected, Tf-CNT-Fe3O4 particles are seen to aggregate following magnetic separation. Figure 4B shows an image of the residual cell suspension containing hPBMC cells and, in this particular case, a single uncaptured HCT116-GFP cell is seen (shown in red circle). Figure 4C shows a plot of recovered vs. spiked HCT116 cells. The % of recovered HCT116 cells for the clinically relevant range of HCT116:hPBMC ratios (1:1 x 104 to 1:1 x 105) are highlighted separately in the histogram of Figure 4D. Cell capture studies confirmed that Tf-CNT-Fe3O4 particles can successfully capture ~ 85% of the cancer cells within 5 min from a background of hPBMNCs even when the abundance of cancer cells is as low as ~0.001% of total number of cells. Experimental Section Preparation of CNT-Fe3O4 microparticle: Purification and oxidation of CNT were carried out using a previously described literature procedure.[18] Oxidized CNTs (AO-CNT) were then mixed with ethylene glycol and water. FeCl3â‹…6H2O and FeCl2â‹…4H2O were added to the mixture and sonicated for 2 h, followed by vigorous stirring for 4 h. The pH was adjusted to ~10 using NH4OH. The resulting CNT-Fe3O4 microparticles were isolated by magnetic separation, washed with distilled water and dried overnight under vacuum. Conjugation of Tf with CNT-Fe3O4: 2 mg of Tf was dissolved in 2 mL of D.I. water and 1.0 mg of the CNT-Fe3O4 was added to it. EDC.HCl was added to this reaction mixture (adding the same number of moles of EDC.HCl as Tf) and constantly stirred at room temperature for 4 h. The reaction time was limited to 4 h to avoid any possible intramolecular cross linking. Resulting Tf conjugated CNT-Fe3O4 microparticles were isolated by magnetic separation and dried at room temperature under vacuum. Tf-CNT-Fe3O4-cell imaging: HCT116-GFP cells were plated at a density of 2 x 105 mL-1 on glass coverslips in 35 mm culture dishes. After 24 h, HCT116 cells in 1 mL DMEM were treated with 500  µg mL-1 of Tf-CNT-Fe3O4 in a NMR tube for 5 min and then subjected to magnetic field separation. The cell pellet obtained after a minute in the strong magnetic field was removed from the remaining cell suspension (containing uncaptured cancer cells). The captured cell pellet and the residual cell suspension was imaged by a Zeiss microscope (Zeiss, Observer.Z1) fitted with a 20 Ãâ€" objective using both bright field and fluorescence channels and the number of cells were counted using ImageJ plugin.[19 Estimation of capture efficiency from artificial CTC suspension: Artificial CTC samples were prepared by spiking hPBMCs with GFP-labeled HCT116 cells at specific ratios (1:103-1:105 for HCT116:hPBMC respectively). 500  µg mL-1 Tf-CNT-Fe3O4 was added to the 1 mL of artificial CTC mixture incubated for 5 min and then subjected to magnetic field separation. The cell pellet obtained after a minute in the strong magnetic field was removed from the remaining cell suspension (containing hPBMCs and uncaptured cancer cells). The captured cell pellet and the residual cell suspension was imaged and the number of HCT116 cells were counted using ImageJ cell counter plugin.[19 Motion parameters, cell culture, isolation of human hPBMC and characterization are included in the supporting information. Conclusions We demonstrated a novel CNT based microrocket that propels efficiently by the thrust of O2 bubbles. The new self-propelled microrockets display ultrafast propulsion in aqueous solution as well as in DMEM. The microrocket displayed a driving force of over 231 and 300 pN in DMEM containing 4% H2O2. The speed and the distance travelled by the microrocket can be manipulated by changing the H2O2 concentration. The designed multifunctional microrocket has the ability to (i) rapidly target (~5 min) and efficiently capture (~85%) TfR+ cancer cells from an artificial CTC-like suspension, (ii) magnetic isolation of the captured cells from peripheral blood cells and (iii) subsequent high resolution imaging. We envision that such self-powered micromotors may provide a new and unique approach for rapid and efficient extraction of CTCs from biological fluids and hence for the early diagnosis of cancer and its recurrence.

COP 3530, Discrete Data Structures and Algorithms, Summer 1999, Homework 4 :: UFL Florida Computer Programming Homework

Class Notes: Data Structures and Algorithms Summer-C Semester 1999 - M WRF 2nd Period CSE/E119, Section 7344 Homework #4 -- Due Wed 16 June 1999 : 09.30am -- Answer Key Answers are in blue typeface. * Question 1. Write pseudocode and a diagram that shows how to implement the merge part of the merge-sort algorithm using two stacks (one for each subsequence), and be sure to use the correct ADT operations for stacks. Do not write Java code, or pseudocode for merge-sort. Answer: 1. Put the two sorted subsequences to be merged, denoted by S1 and S2 on stacks of the same name. Assume that the sorting is in ascending order; hence, each stack has its minimum values at the top of the stack. 2. The merge algorithm proceeds as follows: { repeat until S1 or S2 is empty { v1 = pop(S1) v2 = pop(S2) output(min(v1,v2)) if (max(v1,v2) = v1) then push v1 onto S1 else push v2 onto S2 endif }