Saturday, March 23, 2019

Applying Staining Techniques to View and Identify Bacteria Essay

AbstractThe main objective of this lab was to identify disparate bacterium by simple, negative, and yard staining. To view each bacteria cell, the bacteria was transferred aseptically to a slide, and they were then viewed by using oil immersion, by a light microscope. From this lab, it was determined that E. coli and B. megaterium are gram negative and B. subtilis and S. Marcesans are gram positive.IntroductionThe purpose of this lab was to view the different characteristics of bacteria by applying various staining techniques. It is important to know the make up if a certain bacteria so an antibiotic may be engineered to repeal the bacteria. From the gram stain, it was possible to determine which bacteria was gram positive or gram negative. This is important because gram-negative bacteria are generally to a greater extent toxic (due to the lipopolysaccaride) are resistant to antibiotics than the gram-positive bacteria.MethodsThe materials used for this lab were1.A light micr oscope2.Four glass slides3.And inoculating loop4.A bunsen burner Burner5.Bacteria (E.coli, S...

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